Course


By Amanda Rose Martinez

The Neural Systems and Behavior course (NS&B) was already humming with activity last week as it prepared to kick off its 33rd year. Setting up equipment in Loeb Laboratory on Wednesday was a group of the course’s seasoned regulars, including faculty Lidia Szczupak and Ron Calabrese and teaching assistant Michael Wright.

His eyes glued to a small, circular level, Wright, who is in his fourth year with the course, meticulously adjusted the legs of what could be called the pièce de résistance of the NS&B’s lab equipment: the floating air table.

Michael Wright (L) of Emory University and Herb Luther of MBL Education precision the alignment of an air table in the NS&B lab. Photo by Amanda Martinez

“Very minute vibrations on a preparation that has two electrodes on neurons can just be the end of the experiment,” says Szczupak, who is from the Universidad de Buenos Aires and is marking her 11th summer with NS&B.

Photo by Amanda Martinez

To create a flawlessly stable surface, each table is covered with a metal plate that rests on top of a pocket of air. Everything—microscopes, cells, electrodes, micromanipulators—is affixed to the plate through a series of metal holes on its surface. This way, any vibrations from the footsteps of people walking around or a slammed door are evenly distributed throughout the sensitive experiment’s components.

Come 9 AM Monday this week, 20 students from around the world began the first of four two-week cycles, each of which explores the neural system of a different animal. First up is the leech, which, with its simple nervous system and neurons that are easily recognized with a microscope, is an ideal starting organism.

Lidia Szczupak. Photo by Amanda Martinez

“The goal is to get the kids to approach live neurons with their own hands,” says Szczupak. Come back in two days, she says, and “you’ll see people who never worked with a single neuron before. They’ll be familiar with putting electrodes in the cells, recording resting potential, action potential, and learning what this means.”

If there’s one event in this cycle that NS&B faculty are eagerly anticipating, it’s an appearance by John Nicholls, “one of the ‘Fathers of the Leech,’ ” as Szczupak calls him. Nicholls, formerly of SISSA in Italy and lately teaching in countries around the world, will give a talk on the significance of the leech in neurophysiology research. No stranger to NS&B, Nicholls can be identified among the instructors in the course’s earliest class photos, which are posted in the hall outside the lab.

John Nicholls is 3rd row, 2nd from right (sleeves rolled up) in the 1979 NS&B class photo.

Veteran NS&B faculty member Ron Calabrese. Photo by Amanda Martinez

But when asked what they’re most looking forward to and consequently, the thing that keeps them coming back year after year, NS&B faculty unanimously cited two things—camaraderie and friendships, old and new. “It’s just a lot of fun,” says Ron Calabrese of Emory University, who joined the course as a TA in 1979 and has been back every year since. “I like meeting people. I like seeing what they’re about.”

“We’ve been on the same team with minor differences for ten years,” Szczupak says. “We became friends and it’s very nice to be together doing this.”

By Beth Liles

Two undergraduates on the MBL campus are excitedly preparing to present talks on their summer research tomorrow: Diara Townes and Victoria Morgan, both students in the Woods Hole Partnership Education Program (PEP).

Now in its second year, PEP immerses undergraduates in the rich scientific and academic culture of the Woods Hole research community, and introduces them to leaders and colleagues in their fields of interest. Sixteen PEP students are being mentored in several Woods Hole institutions this summer, including Townes, who is from Hampton University, and Morgan, from Cornell.

PEP student Diara Townes works with MBL veterinarian Amy Hancock in the Marine Resources Center. Photo by Tom Kleindinst

PEP student Diara Townes works with MBL veterinarian Amy Hancock in the Marine Resources Center. Photo by Tom Kleindinst

“This has been the most exciting summer in my undergraduate career,” says Townes. “Not only am I gaining exquisite research experience, but I am also making lasting friendships and amazing professional connections.”

Designed primarily for juniors and seniors interested in marine and environmental science, the PEP curriculum consists of a four-week course followed by six-to-eight week individual research projects. The students are presenting their research findings tomorrow, August 13, from 8:30 AM to 1 PM in the Tilley Conference Room, U.S. Geological Survey Woods Hole Science Center, 384 Woods Hole Road (Quissett Campus), Woods Hole.

MBL veterinarian Amy Hancock in the Marine Resources Center has been mentoring Townes. They are evaluating a new fish anesthetic on various ornamental species to observe its effects on the animals. Townes is also working with Kristy Owen at NOAA’s Woods Hole Science Aquarium. They are measuring dissolved oxygen (DO) levels in the facility’s three main reservoirs and six exhibit tanks to determine if there is a correlation between DO levels and the fish disease exophthalmia (“pop-eye”).

Morgan is working with Ecosystems Center scientist Jim Tang, testing the method to measure carbon dioxide emissions from soils and measuring stem respiration from the Center’s Harvard Forest field site. The project goal is to examine the climate change impact on ecosystems and the feedback of ecosystems to the climate.

Victoria Morgan places soil samples into an oven in order to get a dry measurement of the bulk density of the soil. Photo by Tom Kleindinst

Victoria Morgan places soil samples into an oven in order to get a dry measurement of the bulk density of the soil. Photo by Tom Kleindinst

“I’ve learned more about the oceans and climate change than I expected,” Morgan says. “I’ve met lots of wonderful people, and I’ve had experiences that will remain with me for years to come. I plan on spreading the good news to my fellow Cornellians: PEP is a wonderful program and I really want others to participate in it!”

PEP is a partnership between the University of Maryland Eastern Shore (UMES) and the Woods Hole Diversity Initiative, a multi-institutional effort to promote diversity in the Woods Hole science community. Members of the Diversity Initiative include NOAA Northeast Fisheries Science Center (NEFSC), Woods Hole Oceanographic Institution, USGS Woods Hole Science Center, SEA, the MBL, and Woods Hole Research Center.

The science institutions in Woods Hole are committed to building a diverse and inclusive community. People from all cultures and all backgrounds can feel welcome in Woods Hole, whether they are visiting, spending a season as students, or spending part or all of their careers working here. PEP is just one of many Woods Hole Diversity Committee efforts. To learn more, go to http://www.woodsholediversity.org/

2010 PEP students and directors: Top Row:  Zachary Williams, George Liles (PEP Program coordinator, NEFSC), Delawrence Sykes, Alexander DeLeon, Nam Siu.  Row 2: Lucy Flores, Emily Motz, Christopher Cepero, Brian Redding, Ambrose Jearld (PEP Program director, NEFSC).  Row 3: Joniqua Howard, Lane Boyer, Melika Uter, Rachel Rochon, Victoria Morgan  Bottom Row: Angela Anorve, Delores Toledo, Anna-Mai Christmas, and Diara Townes. Photo by Sateesh Rogers

2010 PEP students and directors: Top Row: Zachary Williams, George Liles (PEP Program coordinator, NEFSC), Delawrence Sykes, Alexander DeLeon, Nam Siu. Row 2: Lucy Flores, Emily Motz, Christopher Cepero, Brian Redding, Ambrose Jearld (PEP Program director, NEFSC). Row 3: Joniqua Howard, Lane Boyer, Melika Uter, Rachel Rochon, Victoria Morgan Bottom Row: Angela Anorve, Delores Toledo, Anna-Mai Christmas, and Diara Townes. Photo by Sateesh Rogers

EllenWalker

Ellen M. Walker, a student in SPINES (Summer Program in Neuroscience, Ethics and Survival) from University of Texas at El Paso, snapped this shot of her workspace at the MBL. Her story behind the photo:

“Throughout my summer here at MBL–whether it was preparing for a presentation after one of my course modules, reading about myosin Va and its action in the neuron for my independent study, or looking for journals the old-fashioned way, in the stacks, and marveling at finding the first volume of Nature and getting caught up in words like ‘whoso’ or ‘hitherto’–this desk on the fifth floor of Lillie has given me a sense of academic solace I cannot adequately or eloquently put into words.”

As a graduate student at University of Texas, Walker is studying how the brain affects behavior in nicotine and alcohol addiction. At the MBL, she has been working with Roy Larson of University of Sao Paolo, a visiting researcher in the Cellular Dynamics Program. Her independent project investigates the visual processing center in the brain of the migratory squid, Loligo pealei.

Duncan Mitchison-Field by Susana Montenegro Gouveia

Susana Montenegro Gouveia, a student in the Physiology course, is the winner of @MBL’s “Photo of the Week” contest. Susana won a free ticket to the MBL Gala concert, featuring the Tokyo String Quartet, this Sunday at 8 PM in Lillie Auditorium. Congratulations, Susana!

Gouveia’s photo shows Duncan Mitchison-Field, son of MBL visiting investigators Tim Mitchison and Christine Field of Harvard Medical School, engrossed in his own intellectual pursuits while his parents help guide Physiology students through their lab work.

“Sometimes (Chris Field) brings the kids with her to the lab,” says Gouveia. “They adapt so nicely to the lab environment, as though they were at home. Duncan was just seated in a corner of the lab, very focused on reading his own book. He was there for hours, so concentrated that he didn’t notice me taking his picture…It really seems that love for reading and acquiring new knowledge is part of his family. Duncan is very young but already shows lots of curiosity, and I wouldn’t be surprised if he were a great scientist in the future.”

@MBL welcomes submissions of photos to be considered for posting as “Photo of the Week.” Please send your photo, along with a caption that identifies any people in the photo (names correctly spelled) to: mblnews@mbl.edu.

Betzig2Friday was the last day MBL Physiology and Neurobiology students had Eric Betzig’s latest invention to explore. Two weeks ago, Betzig (left) brought his new contribution to high-resolution microscopy to the MBL from his lab at HHMI’s Janelia Farm campus and installed it in the central microscopy facility on the ground floor of Loeb.

“It’s really at the very bleeding edge,” Betzig says of his microscope, “but it’s a really good time to bring it (to the MBL), where all sorts of world-class cell biologists throw everything they can think of at it. We can learn through trial by fire what works and what doesn’t.”

The microscope combines two concepts—plane illumination microscopy and Bessel beams—to allow for high-resolution imaging within live cells. Cell components are labeled with fluorescent markers, and excitation of these markers by specific wavelengths of light allows them to be visualized by the microscope. (See video below to hear Betzig explain how his Bessel beam plane illumination microscope works).

The microscope is the just latest invention that Betzig has test-run at the MBL. In 2007, Betzig loaded his cutting-edge PALM (photoactivated localization microscopy) equipment into the back of an SUV and drove it up to the Physiology course, upon the invitation of Physiology faculty member and NIH senior investigator Jennifer Lippincott-Schwartz. PALM allows scientists to discriminate molecules only two to 25 nanometers apart, a vast improvement on the previous 200-nanometer limitation. At the MBL, Betzig, Lippincott-Schwartz, Jim and Cathy Galbraith of the NIH, Hari Shroff, a former Physiology student, and students then in the course worked feverishly around the clock with PALM. “By the end of two weeks, we had gotten PALM to work with live cells for the first time!” says Lippincott-Swartz. Not only that, but they got PALM to work with two colors of fluorescent probes rather than one, and demonstrated that it could be used to track single molecules in live cells. “It was a spectacular session, and it led to several publications,” Lippincott-Swartz says.

What’s next for Betzig? “I don’t know how many times we’ll invent a new microscope,” Betzig says, “but when we do, the MBL is a good place to be for that kind of intense trial to try to find out what works.”

Janelia Farms/HHMI scientists Thomas Planchon, Research Specialist; Eric Betzig, Group Leader; and Liang Gao, Postdoctoral Scientist, have been testing a new microscope with the help of MBL students and faculty.

Janelia Farm/HHMI scientists Thomas Planchon, Research Specialist; Eric Betzig, Group Leader; and Liang Gao, Postdoctoral Scientist, have been testing a new microscope with the help of MBL students and faculty.

Eric Betzig works on the new microscope.

Eric Betzig works on the new microscope.

Betzig discusses the new Bessel beam plane illumination microscope:

In this clip, Betzel’s microscope allows for visualization of histones – proteins associated with DNA – inside a pig kidney cell:

Here, the microscope reveals a network of mitochondria – cellular components that synthesize ATP, the “fuel” of the cell – inside a human cancer cell:

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